Figure 2.

RBO protein is subcellularly restricted to synapses. Confocal imaging of RBO-eGFP in rbo transgenic rescue background (rbo²/rbo²; rbo-egfp/rbo-egfp). a, RBO at wandering third instar larva NMJ synapses. Colocalization of RBO with synaptic markers is shown (DLG, predominantly postsynaptic and CSP, a presynaptic vesicle-associated protein). b, RBO in adult abdominal NMJs. Anti-HRP recognizes the neuronal membrane. c, Innervation pattern of DLM NMJs stained with anti-synaptotagmin. NMJs are deeply embedded and evenly distributed in DLM muscle cells. The bottom is the projection of a single DLM muscle cell. d, e, Lower amplification (d) and higher amplification (e), showing that RBO colocalizes with HRP in DLM NMJs.