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Conditional block of synaptic vesicle exocytosis in rbo mutants. a, Representative electron micrograph of a DLM NMJ synaptic bouton from wt at room temperature (22°C). b, Representative bouton of rbo mutant (rbots1/rbo2) at 22°C. Note that SVs tend to be distributed toward active zones. c, Representative bouton of rbo mutant after a 10 min shift to 37°C. Note dramatic increase in SV density. d, Quantification of SV number per bouton profile. The bars show the mean ± SEM.
