Fig. 5.

Effect of temperature and sucrose on primary root growth of Arabidopsis wild-type (WT), deg10-2, deg10-1, and catr3-1 seedlings. Per genotype and condition, 54 seeds (from three different parental plants) were plated on medium with or without 2% sucrose. All plates were incubated at ambient temperature (21 °C day/17 °C night) for 3 days to allow germination and seedling establishment. Thereafter, the plates for temperature stress were incubated at elevated temperature (30 °C day/25 °C night), and after 13 days the length of the primary root was measured. Between 27 and 54 plants per genotype and condition showed continuous growth during this period; all others were excluded from the analysis. Boxes show the interquartile range with medians, whiskers extend to the most extreme data point within 1.5 times the interquartile range, and open circles indicate data points outside this range. Boxes that are not marked by a common letter have significantly different means (P<0.05 in all-pairwise comparisons of log-transformed values with Tukey’s honestly significant difference post-hoc test). Data are from one out of three experiments with similar results. See Supplementary Table S5 for the analysis of interactions between genotype and treatment.