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. 1997 Jan 1;17(1):23–31. doi: 10.1523/JNEUROSCI.17-01-00023.1997

Fig. 4.

Fig. 4.

CR-50 epitope mapping. Plasmids encoding truncated Reelin proteins were transcribed in vitro by the T7 RNA polymerase and translated by using a rabbit reticulocyte lysate in the presence of [35S]methionine and [35S]cysteine. A, The products of pCrl1837 (206 kDa, lane 1), pCrl462 (51 kDa, lane 2), pCrl407 (46 kDa, lane 3), pCrl250 (27.5 kDa,lane 4), and pCrl194 (22 kDa, lane 5) were analyzed on a 4–12% SDS-polyacrylamide gel.B, The same in vitro translated proteins as in A were subjected to immunoprecipitation with the monoclonal antibody CR-50 and analyzed on a 4–12% SDS-polyacrylamide gel. Because all truncated proteins except those encoded by pCrl194 and pCrl250 are recognized by CR-50, the epitope is located between amino acids 251 and 407 of Reelin.