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. 1997 Jan 1;17(1):227–240. doi: 10.1523/JNEUROSCI.17-01-00227.1997

Fig. 5.

Fig. 5.

GluR1 mRNAs are neither expressed nor regulated by growth factors in CG-4 cells. A, RT-PCR analysis of GluR1 transcripts in O-2A and CG-4 cells cultured in N1 + 0.5% FBS (N1), in PDGF+bFGF (P+F; 10 ng/ml each), and in B104-conditioned medium (B104). Negative controls included no RT reaction to exclude genomic DNA contamination (no RT) and no template in the RT reaction to exclude reagent contamination (no templ). The expected RT products (700 bp) were obtained from O-2A cells cultured inN1, PDGF+bFGF (P+F), or B104, but not from CG-4 cells. B, Northern blot analysis of total RNA isolated from CG-4 cells cultured in N1 + 0.5% FBS (N1), in PDGF+bFGF (P+F; 10 ng/ml each), and in B104-conditioned medium (B104). The blot was hybridized with a GluR1 KpnI cDNA probe. P1 cortex, Positive control. Treatment with PDGF+bFGF, which strongly upregulates GluR1 RNA expression in O-2A cells, did not stimulate GluR1 expression in CG-4 cells. This blot was overexposed (2 weeks) to detect low levels of GluR1 transcripts.