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SV40 T antigen (Tag) is lost in CAD cells.A, Western blot analysis with Tag-specific polyclonal antibody. Each lane contains 50 μg of protein. Tag was detected in Cath.a cells (CA) as a doublet. No Tag was detected in CAD cells. B, Southern blot analysis with a32P-labeled Tag probe. Tag is only detected in Cath.a cells, but not in CAD or PC12 cells. PC, PC12 cells;KD, kilodalton.
