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Representative Northern blot showing that single acute i.c.v. injections of LPS (1) had greater effects on brain TNF-α mRNA abundance than injections that were preceded 5 d earlier by a similar i.c.v. injection (2). Ethidium bromide staining of agarose gel demonstrated equal loading of samples. Representative of two experiments: 2 d exposure, −70°C, two intensifying screens.
