Fig. 3.

Neuroprotective activity of the glial-conditioned medium collected from pure cultures of astrocytes, at different times after a 10 min exposure to DCG-IV (1 μm). Values were obtained from a representative experiment and calculated from the mean of four individual determinations. Values relative to the glial-conditioned medium collected at 2, 6, or 20 hr after exposure to DCG-IV were significantly different as compared with values obtained with control (CTRL) astrocyte medium (i.e., with medium from astrocytes treated with the buffer alone) or with the medium collected from astrocyte cultures immediately after washing out DCG-IV (labeled as 0) (p < 0.01 by one-way ANOVA + Fisher PLSD test). This experiment was repeated two times with similar results.