Table 1.

Neuroprotective activity of mGlu receptor agonists against NMDA toxicity in mixed cultures of cortical cells

	Count of dead cells		LDH release
	Control	NMDA (100 μm)	Control	NMDA (100 μm)
Basal	36  ± 6	415  ± 15	21  ± 6	245  ± 9
DCG-IV, 1 μm	29  ± 8	163  ± 6*	31  ± 5	100  ± 6*
4C3HPG, 50 μm	42  ± 12	205  ± 7*	19  ± 7	120  ± 5*
PCCG-IV, 20 μm	28  ± 4	385  ± 22	17  ± 9	261  ± 10
DCG-IV + PCCG-IV	31  ± 6	395  ± 10	24  ± 11	240  ± 8
4C3HPG + PCCG-IV	31  ± 5	380  ± 7	17  ± 5	210  ± 8
l-CCG-I, 10 μm	40  ± 6	197  ± 8*	15  ± 7	115  ± 7*
l-AP4, 10 μm	26  ± 4	223  ± 5*	17  ± 8	137  ± 6*

mGlu receptor agonists and/or PCCG-IV were applied in combination with NMDA. Values are means ± SEM of 6–15 determinations.

^*p < 0.01 (one-way ANOVA + Fisher protected least significant difference), when compared with NMDA alone. Dead cells were counted as cells stained with trypan blue in three random fields/well (200 cells counted per field). LDH release is expressed as mOD/min. LDH, lactate dehydrogenase.