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. 1997 Mar 15;17(6):2006–2017. doi: 10.1523/JNEUROSCI.17-06-02006.1997

Fig. 2.

Fig. 2.

Digitized confocal microscopic images of NMDAR1 (B, D, F,H, J, L, N,P) and synaptophysin (SYN;A, C, E, G,I, K, M, O) immunofluorescence in the dentate gyri of an unoperated control animal (Ctrl, A–D) and animals examined 2 (E–H), 5 (I–L), and 9 (M–P) d postlesion. The series on the left represent fields from the nonlesioned, contralateral side; the series on theright represent fields from the lesioned, ipsilateral side. Note that the pattern of synaptophysin-positive puncta is uniform across the entire molecular layer (OML andIML) in both sides of the control animal (A, C) and in the contralateral side of the 2 d (E), 5 d (I), and 9 d (M) animals. In contrast, an overt decrease in synaptophysin immunofluorescence is evident in the OML compared with the IML on the ipsilateral side in the 2 d (G), 5 d (K), and 9 d (O) animals. NMDAR1 immunofluorescence is relatively uniform throughout the entire molecular layer (OML andIML) in the control animal (B,D) and the contralateral side of all the lesioned animals (F, J, N). In the 2 d postlesion animal, a similar, homogeneous pattern is evident across the molecular layer on the ipsilateral side (H). In contrast, an overt increase in the immunofluorescence intensity of the OML compared with the IML is apparent in the ipsilateral side of the animals 5 (L) and 9 d (P) postlesion. Scale bar, 30 μm.