Skip to main content
. 1997 Mar 15;17(6):2056–2070. doi: 10.1523/JNEUROSCI.17-06-02056.1997

Fig. 4.

Fig. 4.

A, B, An OL in a primary culture (20 DIV), treated with 23-mer antisense to PLP, 2.5 μm, beginning at 13 DIV. This cell is (A) MBP+ and (B) PLP−. The cell body (asterisk) has several membrane sheets visualized with MBP immunostaining. The patchy staining of the membrane sheets seen here using antibodies to myelin proteins is attributable to treatment of the cells with Triton X-100, which is required to maximize immunostaining for myelin proteins. Magnification, 450×.C, Phase-contrast micrograph of an enriched culture treated with 23-mer PLP antisense for 10 d beginning 3 d after enrichment. Several phase-bright cells with long processes are characteristic features of OLs visualized with phase-contrast microscopy. The length of their processes and the extensive branching of a few of these cells are characteristic of healthy OLs.