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. 1997 Mar 15;17(6):2056–2070. doi: 10.1523/JNEUROSCI.17-06-02056.1997

Fig. 5.

Fig. 5.

Shown are OLs in a primary culture incubated with S-ODNs to PLP beginning at 13 DIV and continuing for 7 d (20 DIV); they were immunostained for MBP (A, B) and PLP (C, D). An OL in a control culture is positive for both MBP (A) and PLP (C). The intense staining of the OL cell body and light staining of its processes and membrane sheets are a consistent finding using PLP antibodies. Note the differential immunostaining pattern using MBP and PLP antibodies. OLs treated with 23-mer antisense to PLP for 7 d are strongly positive for MBP (B) but lack PLP staining (D). The vast majority of the OLs in the antisense-treated cultures typically lacks membrane sheets and appears immature. Magnification, 450×.