Fig. 1.

Specificity of the oligonucleotide probes forLmo1, Lmo2, and Lmo3 was confirmed byin situ hybridization competition studies. Sections through the forebrain of mice injected with KA (6 hr survival) were processed for in situ hybridization such that each of the three ³⁵S-labeled sequences was incubated either alone or in competition with 100-fold excess of each of the three unlabeled oligonucleotide sequences. Autoradiographs of the hybridization signals demonstrated that specific hybridization signal was prevented only by coincubation of labeled probes with unlabeled oligonucleotides of the same sequence. Scale bar, 1500 μm.