Fig. 6.

Protein kinase inhibitors H-89 and H-7 prevent effects of 8-CPT-cAMP but do not reduce baseline activity.A, Two sets of five consecutive sweeps of Lp channel activity from a control patch (left) and one that had been pretreated with 1 mm 8-CPT-cAMP plus 1 μm H-89 (right). Both multichannel patches contained only one Lp channel, but the control patch contained three Ls channels, and the treated patch contained two Ls channels. Note that activity of the Lp channel in the patch on the right was similar to Lp activity in the patch on the left, unlike what occurred after treatment with 1 mm 8-CPT-cAMP alone (compare Lp reopenings in sweeps with the multichannel recording in Fig. 2B). B, C, Time course of Lp reopening probability for recordings illustrated in A. Note that activity in 8-CPT-cAMP plus H-89 (C) was similar to that of control (B).D, Probability of Lp reopenings under four different experimental conditions using H-89 (n = 7, 8, 5, and 5 for control, 8-CPT-cAMP, 8-CPT-cAMP plus H-89, and H-89, respectively). E, Same format as C, except for H-7 (n = 4, 7, 6, and 2 for control, 8-CPT-cAMP, 8-CPT-cAMP plus H-7, and H-7, respectively).Asterisks indicate significant differences (p < 0.05).