Table 1.

Ca²⁺ channel mRNA levels in hippocampal neurons

Age in culture	α1D mRNA	α1C mRNA
3 DIV	258  ± 25	1790  ± 103
10 DIV	544  ± 112	3391  ± 713
15 DIV	632  ± 144*	2918  ± 659
28 DIV	680  ± 104*	3606  ± 450

A significant increase in mRNA levels for the α_1Dsubunit of the L-type Ca²⁺ channel was detected as a function of age in culture (*p < 0.05 vs 3 DIV; ANOVA and post hoc Student–Newman–Keuls comparisons test). Values are arbitrary density units for the specific message divided by the total RNA per preparation and are expressed as the mean ± SEM. n = 3–4 samples per time point.