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Time course of Aβ neurotoxicity. Cultured rat cortical neurons were treated with Aβ1–40 (20 μm, Lot ZK840) for the indicated intervals, and cell viability was assayed by metabolic integrity (XTT reduction and alamarBlue reduction) and plasma membrane integrity (LDH release). Metabolic parameters decrease well before the loss of membrane integrity. Data are mean ± SD (error bars) values from triplicate wells and represent typical results obtained with these cells on a routine basis.
