. 1997 Oct 15;17(20):7890–7901. doi: 10.1523/JNEUROSCI.17-20-07890.1997

Table 4.

Regulation of opiate withdrawal by 5 d of intra-LC infusion of antisense oligonucleotide to CREB

	Severity of withdrawal behavior
	Sense-treated		Antisense-treated
	0–15 min	16–30 min	0–15 min	16–30 min
Behaviors attenuated by CREB antisense oligonucleotide
Teeth chatter	2.2 ± 0.3	1.7 ± 0.4	2.4 ± 0.4	0.3 ± 0.2^4-160
Wet dog shakes	2.8 ± 0.3	1.2 ± 0.3	2.2 ± 0.2^4-150	0.7 ± 0.2^4-160
Ptosis	1.4 ± 0.2	1.8 ± 0.2	1.1 ± 0.1	1.1 ± 0.2^4-160
Irritability	1.3 ± 0.2	1.8 ± 0.2	0.9 ± 0.2^4-150	0.7 ± 0.2^4-160
Vacuous chewing	1.6 ± 0.3	1.4 ± 0.4	1.3 ± 0.2	0.7 ± 0.2^4-160
Behaviors not affected by CREB antisense oligonucleotide
Piloerection	1.3 ± 0.2	1.1 ± 0.4	1.3 ± 0.3	1.3 ± 0.2
Lacrimation	0.5 ± 0.2	0.8 ± 0.1	0.8 ± 0.3	0.8 ± 0.1
Salivation	0	0.5 ± 0.3	0	0.5 ± 0.4
Diarrhea	1.2 ± 0.4	1.5 ± 0.4	0.9 ± 0.3	1.2 ± 0.4
Stereotypy	0.7 ± 0.3	0	0.9 ± 0.5	0

Rats received bilateral intra-LC infusions of antisense or sense oligonucleotide to CREB and were then treated chronically with morphine (see Materials and Methods). Withdrawal was precipitated by administration of naltrexone (10 mg/kg, s.c.). Withdrawal behaviors were scored from 0 to 3 as described in Materials and Methods. No withdrawal behaviors were observed in rats before administration of naltrexone (data not shown). Data are expressed as mean ± SEM (n = 6 in each group).

F4-150: p < 0.1;

F4-160: p < 0.05 byt test.