Table 1.
EC50 values of GluR6 and chimeras containing ion pores from KBPs of different species
| Clone | EC50KA | nH | Imax[nA] | n | EC50GLU | nH | Imax[nA] | n |
|---|---|---|---|---|---|---|---|---|
| GluR6(Q) wild type | 1.74 ± 0.72 | 1.09 ± 0.11 | 6224 ± 1487 | 3 | 34.4 ± 6.2 | 1.2 ± 0.12 | 9898 ± 2042 | 3 |
| GluR6(Q)-PCS | 2.2 ± 0.06 | 0.97 ± 0.06 | 667 ± 179 | 4 | 59.9 ± 4.8 | 1.2 ± 0.06 | 695 ± 132 | 3 |
| GluR6-PKBP(Rp) | 2.4 ± 0.15 | 1.4 ± 0.1 | 8.02 ± 1.13 | 4 | 35.7 ± 7.35 | 1.78 ± 0.2 | 6.47 ± 1.9 | 5 |
| GluR6-PKBP(Gd) | 6.0 ± 1.4 | 0.93 ± 0.08 | 6.0 ± 0.7 | 5 | 47.2 ± 1.6 | 1.35 ± 0.02 | 10.61 ± 1.2 | 3 |
| GluR6-PKBP(Ca)α | 2.16 ± 0.05 | 1.64 ± 0.26 | 55.9 ± 16.2 | 4 | 49.9 ± 2.8 | 1.47 ± 0.04 | 58.7 ± 10.8 | 4 |
| GluR6-PKBP(Ca)β | 2.9 ± 0.8 | 1.06 ± 0.1 | 11.03 ± 3.4 | 4 | 35.6 ± 2.3 | 1.26 ± 0.1 | 18.4 ± 5.5 | 4 |
| GluR6-PKBP(XI) | 2.25 ± 0.53 | 0.95 ± 0.04 | 7.9 ± 2.25 | 4 | 44.4 ± 7.5 | 1.1 ± 0.17 | 8.1 ± 1.12 | 3 |
Half-maximal efficient concentrations (EC50) for kainate (KA) and glutamate (Glu), Hill coefficients (nH), as well as maximal current amplitudes (Imax) are listed ± SEM; n, number of oocytes tested. PCS, Clone-engineered for easy pore cassette exchange (containing unique restriction sites flanking the pore; see Materials and Methods for details). GluR6-PKBP(Nn), GluR6 containing the pore domain of a KBP; “Nn” stands for the abbreviation of the Latin names of the species from which the KBP ion pore originated (Rp = Rana pipiens; Gd = Gallus domesticus; Ca = Carassius auratus, genes α and β; XI = Xenopus laevis).