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. 1997 Oct 15;17(20):7881–7889. doi: 10.1523/JNEUROSCI.17-20-07881.1997

Fig. 4.

Fig. 4.

Developmental compartments are disrupted early in the En-2hd/hd mutant. Whole-mount cerebella from En-2hd/hd, L7lacZ(B, D, F, H) stained with β-galactosidase are compared with wild-type mice (A, C, E, G) at different perinatal ages: E18 (A, B), P0 (C, D), and P2 (E, F). At E18, two medial bands are present in both control (A) and mutant (B) animals. By P0, the first disruptions are apparent. In wild-type (C), four bands are located in the region of the eighth and ninth folia, compared with the highly diffuse staining (asterisk) found inEngrailed-2 mutants (D). At P2 (E–H) many differences are noticeable. There is a loss of a midline interband (F, asterisk), the precocious appearance of a stained band in Crus I (F, located under I in the mutant), and the addition of two medial bands in lobule IX (G, compare bandsa, b in wild type with the pattern in theEn-2hd/hd lobules 8, 9,H). S, Simplex.