Fig. 5.
L7lacZ band loss persists into adulthood. Whole-mount cerebella from En-2hd/hd,L7lacZ (B, D, F) stained with β-galactosidase are compared with wild-type mice (A, C, E) at different postnatal ages: P4 (A, B), P7 (C, D), and P30 (E, F). P4 mutants lack lateral bands of transgene activity in vermal lobules III and IV, and staining is generally diffuse (A, B, asterisks). By P7 staining in the Crus II- and pml-fused folia reveals that band width is significantly reduced in the mutant (compare the region identified by the asterisk in C, lobule8, with that in D). Severe band loss is recognizable in the adult mutant (F), most noticeably in the vermis compared with wild type (E). In general, there appears to be less overall reaction product in En-2 mutants at this time.S, Simplex; I, Crus I; II, Crus II.