KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| PD-L1 antibody (clone 28-8 mAb) | Abcam | Cat#ab205921; RRID:AB_2687878 |
| CD274 rabbit polyclonal antibody | Abcam | Cat#ab58810; RRID:AB_940872 |
| PD-L1 (E1L3N) XP rabbit antibody | Cell Signaling Technology | Cat#13684; RRID:AB_2687655 |
| Atezolizumab | MDACC | N/A |
| α-tubulin antibody (monoclonal) | Sigma-Aldrich | Cat#T5168; RRID:AB_477579 |
| Actin antibody | Sigma-Aldrich | Cat#A2066; RRID:AB_476693 |
| Peroxidase-AffiniPure Goat anti-Mouse IgG (H+L) | Jackson ImmunoResearch | Cat#115-035-003; RRID:AB_10015289 |
| Peroxidase-AffiniPure Goat anti-Rabbit IgG (H+L) | Jackson ImmunoResearch | Cat#111-035-003; RRID:AB_2313567 |
| Goat Anti-Rabbit IgG (H+L) antibody, Fluorescein Conjugated | Thermo Fisher Scientific | Cat#F2765; RRID:AB_10562896 |
| Bacterial and Virus Strains | ||
| N/A | ||
| Biological Samples | ||
| Multi-organ carcinoma tissue array | Biomax | Cat#BC000119 |
| Lung cancer tissue array | Biomax | Cat#NSC151 |
| Rectal cancer tissue array | This paper | N/A |
| Human pre-immunotherapy archived tissue samples | This paper | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| PNGase F | New England BioLabs | Cat#P0704 |
| AEC chromogen substrate (3-amino-9-ethylcarbazole) | Sigma-Aldrich | Cat#A5754 |
| N,N-dimethylformamide | Sigma-Aldrich | Cat#D4254 |
| Aqua-mount mounting medium | DAKO | Cat#S1964 |
| ProLong™ Gold Antifade Mountant with DAPI | Invitrogen | Cat#P36931 |
| TMB Substrate | Cell Signaling Technology | Cat#7004 |
| TMB High Sensitivity Substrate Solution | BioLegend | Cat#421501 |
| STOP Solution | Cell Signaling Technology | Cat#7002 |
| Stop Solution for TMB Substrate | BioLegend | Cat#423001 |
| BSA blocking solution for ELISA | Santa Cruz Biotechnology | Cat#293965 |
| Animal-Free Blocking Solution | Cell Signaling Technology | Cat#15019 |
| Concanavalin A, HRP-conjugated | Sigma-Aldrich | Cat#L6397 |
| Critical Commercial Assays | ||
| N/A | ||
| Deposited Data | ||
| N/A | ||
| Experimental Models: Cell Lines | ||
| BT-549 | ATCC | ATCC#HTB-122 |
| BT-20 | ATCC | ATCC#HTB-19 |
| MDA-MB-231 | ATCC | ATCC#HTB-26 |
| MCF-7 | ATCC | ATCC#HTB-22 |
| H1437 | ATCC | ATCC#CRL-5872 |
| A549 | ATCC | ATCC#CCL-185 |
| Calu3 | ATCC | ATCC#HTB-55 |
| H1299 | ATCC | ATCC#CRL-5803 |
| H1355 | ATCC | ATCC#CRL-5865 |
| H358 | ATCC | ATCC#CRL-5807 |
| H1435 | ATCC | ATCC#CRL-5870 |
| H226 | ATCC | ATCC#CRL-5826 |
| H322 | ATCC | ATCC#CRL-5806 |
| Jurkat | ATCC | ATCC#TIB-152 |
| THP1 | ATCC | ATCC#TIB-202 |
| Experimental Models: Organisms/Strains | ||
| N/A | ||
| Oligonucleotides | ||
| N/A | ||
| Recombinant DNA | ||
| N/A | ||
| Software and Algorithms | ||
| Prism (version 7) | GraphPad | N/A |
| Other | ||
| N/A | ||