Skip to main content
. 2019 Oct 15;14(10):e0213630. doi: 10.1371/journal.pone.0213630

Fig 2. (pp)pGpp alarmone synthesis activities of Sa-RelP and Sa-RelQ.

Fig 2

Panels A−I show representative anion-exchange chromatograms performed under analogous conditions (as described in Materials and methods). Panels A−C show mixtures of pure nucleotides (A) ATP, ADP and AMP, (B) GTP, GDP and GMP, (C) pGpp, ppGpp and pppGpp. Panels D−F show product mixtures formed by the incubation of the Sa-RelQ protein with the following nucleotide mixtures: (D) ATP + GTP, (E) ATP + GDP, (F) ATP + GMP. Panels G−I show product mixtures formed by the incubation of the Sa-RelP protein with the following nucleotide mixtures: (G) ATP + GTP, (H) ATP + GDP, (I) ATP + GMP.