Skip to main content
. 2019 Oct 15;14(10):e0213630. doi: 10.1371/journal.pone.0213630

Fig 4. V0/[S] plots used to calculate enzymatic kinetic parameters for (pp)pGpp-stimulated (pp)pGpp-synthesis activities of Sa-RelQ.

Fig 4

Panels A−I show plots of rate of (pp)pGpp synthesis (V0; Y-axis, in units of μM / minute) versus GTP/GDP/GMP substrate concentration ([S]; X-axis; in micromolar units), for sets of assays performed to calculate the enzymatic kinetic parameters for the Sa-RelQ protein. All assays were performed in the presence of 100 μM concentrations of pppGpp, ppGpp or pGpp stimulatory alarmone, as indicated in the respective panels. (Panels A−C) pppGpp synthesis in the presence of pppGpp (A), ppGpp (B), or pGpp (C). (Panels D−F) ppGpp synthesis in the presence of pppGpp (D), ppGpp (E), or pGpp (F). (Panels G−I) pGpp synthesis in the presence of pppGpp (G), ppGpp (H), or pGpp (I). Experimental details are described in the materials and methods section.