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. 2019 Oct 15;14(10):e0213630. doi: 10.1371/journal.pone.0213630

Fig 5. V0/[S] plots used to calculate enzymatic kinetic parameters for (pp)pGpp synthesis activities of Sa-RelP.

Fig 5

Panels A−C respectively show the plots of rate of pppGpp, ppGpp and pGpp synthesis (V0; Y-axis, in units of μM / minute) versus GTP, GDP, or GMP substrate concentrations ([S]; X-axis; in micromolar units), for sets of assays performed to calculate the enzymatic kinetic parameters for the Sa-RelP protein. Experimental details are described in the materials and methods section. 2–3 replicates were performed for each condition, with mean values plotted ± standard deviation.