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. 2019 Oct 15;14(10):e0223703. doi: 10.1371/journal.pone.0223703

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© 2019 Takei et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 5 — A: Localization of mRNA encoding the βA subunit in kidney specimens of patients with renal AAV was examined by in situ hybridization. Hybridization signals are shown as blue color. Magnification, ×1000. (a) normal glomeruli, (b, d) crescentic glomeruli, (c) tubules. Detection using (a-c) anti-sense probe or (d) sense probe (control). B: Colocalization of activin A βA subunit and LTL in normal kidney specimens obtained from patients who underwent nephrectomy because of renal cancer. βA subunit (red), LTL (green) and DAPI (blue). Magnification, ×400. C, D: Colocalization of βA subunit for activin A and CD68 in glomeruli (B) and interstitium (C) of kidney specimens of patients with renal AAV. βA subunit (green), CD68 (red), DAPI (blue). Magnification, ×400. E: Colocalization of activin A βA subunit and LTL in kidney specimens of renal AAV patients. βA subunit (red), LTL (green) and DAPI (blue). Magnification, ×1000.