Key resources table.
| Reagent type | Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (D. melanogaster) | clk856-GAL4 | Gummadova et al., 2009 | Flybase: FBtp0069616 | |
| Genetic reagent (D. melanogaster) | UAS-Kir2.1 | Bloomington Drosophila Stock Center | BDSC_6595 | |
| Genetic reagent (D. melanogaster) | pdf-GS-GAL4 | Depetris-Chauvin et al., 2011 | FBal0267534 | |
| Genetic reagent (D. melanogaster) | per01 | Konopka and Benzer, 1971 | FBal0013649 | |
| Genetic reagent (D. melanogaster) | mai179-GAL4 | Grima et al., 2004 | FBal0124017 | |
| Genetic reagent (D. melanogaster) | MB122B-GAL4 | Guo et al., 2017 | ||
| Genetic reagent (D. melanogaster) | pdf-GAL4 | Renn et al., 1999 | FBtp0011844 | |
| Genetic reagent (D. melanogaster) | clk4.1M-GAL4 | Zhang et al., 2010 | FBtp0054012 | |
| Genetic reagent (D. melanogaster) | UAS-Cas9.P2 | Bloomington Drosophila Stock Center | BDSC_58986 | |
| Genetic reagent (D. melanogaster) | AstC-GAL4 | Bloomington Drosophila Stock Center | BDSC_52017 | |
| Genetic reagent (D. melanogaster) | dvPDF-GAL4 | Guo et al., 2014 | FBtp0081543 | |
| Genetic reagent (D. melanogaster) |
w;CyO/Sco;
MKRS/TM6B |
Bloomington Drosophila Stock Center | BDSC_3703 | |
| Genetic reagent (D. melanogaster) | VGlut-GAL4 | BloomingtonDrosophila Stock Center | BDSC_60312 | |
| Genetic reagent (D. melanogaster) | GMR-ss00650-GAL4 | G Rubin, Janelia Research Campus | ||
| Genetic reagent (D. melanogaster) | GMR-ss01038-GAL4 | G Rubin, Janelia Research Campus | ||
| Genetic reagent (D. melanogaster) | GMR-ss00849-GAL4 | G Rubin, Janelia Research Campus | ||
| Genetic reagent (D. melanogaster) | GMR-ss00367-GAL4 | G Rubin, Janelia Research Campus | ||
| Genetic reagent (D. melanogaster) | GMR-ss00681-GAL4 | Liang et al. (2019) | ||
| Genetic reagent (D. melanogaster) | GMR-ss00645-GAL4 | G Rubin, Janelia Research Campus | ||
| Genetic reagent (D. melanogaster) | GMR-GAL4 | BloomingtonDrosophila Stock Center | BDSC_1104 | |
| Genetic reagent (D. melanogaster) | repo-GAL4 | BloomingtonDrosophila Stock Center | BDSC_7415 | |
| Genetic reagent (D. melanogaster) | tub-GAL80ts | Bloomington Drosophila Stock Center | BDSC_7018 | |
| Genetic reagent (D. melanogaster) | UAS-per-g | This study | ||
| Recombinant DNA reagent | pCFD6 | Addgene | RRID: Addgene73915 | |
| Software | Fiji | https://fiji.sc/ | ||
| Software | Stata SE15 | |||
| Software | Microsoft Office Excel | |||
| Software | ActogramJ | actogramj.neurofly.de | ||
| Antibody | Anti-PER Rabbit polyclonal |
Stanewsky et al., 1998 | 1:1000 | |
| Antibody | Anti-PDF Mouse monoclonal |
Developmental Studies Hybridoma Bank | AB_760350 | 1:500 |
| Antibody | Anti-PDP1 Guinea pig polyclonal |
Benito et al., 2007 | 1:2000 | |
| Antibody | Goat anti-mouse polyclonal | ThermoFisher | A-31575 | 1:200 |
| Antibody | Goat anti-rabbit polyclonal | ThermoFisher | A-11034 | 1:200 |
| Antibody | Goat anti-rabbit polyclonal | ThermoFisher | A-32732 | 1:200 |
| Antibody | Goat anti-guinea pig polyclonal | ThermoFisher | A-11073 | 1:200 |
| Commercial assay or kit | MIDI-Prep Kit | Qiagen | 12143 | |
| Commercial assay or kit | Q5 Polymerase | NEB | M0491S | |
| Commercial assay or kit | BbSI-Enzyme | NEB | R0539S | |
| Commercial assay or kit | Vectashield | Vectorlabs | H-1000 |