Transgene | gRNAs expressed (orientation of target sequence) |
---|---|
UAS-t:sgRNA-per4x | 1. GCTTTTCTACACACACCCGG (5’→3’) 2. CACGTGCGATATGATCCCGG (3’→5’) 3. GGAGTCCACACACAACACCA (5’→3’) 4. TACTCGTCCATAGACCACGC (5’→3’) |
UAS-t:sgRNA-tim3x | 1. *TCTGCTGAAGGAATTCACCG (5’→3’) 2. TGTGGCGACCCACATCCGTG (3’→5’) 3. GAGAACGCGCTGTACAACTG (3’→5’) 4. AAGAGGCCAGCGATATGACG (5’→3’) |
UAS-t:sgRNA-acp98AB4x | 1. GTGTCCCCTTATTCGTGCGG (3’→5’) 2. CACACTATCAAAGGATGACG (5’→3’) 3. ATAAGGGGACACACTATCAA (5’→3’) 4. AGTGTGTCCCCTTATTCGTG (3’→5’) |
*sgRNA scaffold for gRNA 1 of timeless has a one bp deletion (GTTTA... instead of GTTTTA...).