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. 2019 Oct 15;8:e48308. doi: 10.7554/eLife.48308
Transgene gRNAs expressed (orientation of target sequence)
UAS-t:sgRNA-per4x 1. GCTTTTCTACACACACCCGG (5’→3’)
2. CACGTGCGATATGATCCCGG (3’→5’)
3. GGAGTCCACACACAACACCA (5’→3’)
4. TACTCGTCCATAGACCACGC (5’→3’)
UAS-t:sgRNA-tim3x 1. *TCTGCTGAAGGAATTCACCG (5’→3’)
2. TGTGGCGACCCACATCCGTG (3’→5’)
3. GAGAACGCGCTGTACAACTG (3’→5’)
4. AAGAGGCCAGCGATATGACG (5’→3’)
UAS-t:sgRNA-acp98AB4x 1. GTGTCCCCTTATTCGTGCGG (3’→5’)
2. CACACTATCAAAGGATGACG (5’→3’)
3. ATAAGGGGACACACTATCAA (5’→3’)
4. AGTGTGTCCCCTTATTCGTG (3’→5’)

*sgRNA scaffold for gRNA 1 of timeless has a one bp deletion (GTTTA... instead of GTTTTA...).