Figure 8. Viral sensing of by natural TRIM21 variants.

(A) Stable 293T cell lines expressing TRIM21 variants were infected with AdV5 in the presence of anti-hexon 9C12 antibody and immune activation was measured 6 hr post infection using an NF-κB luciferase reporter. Data compiled from at least two independent experiments and expressed as fold change over that of virus only condition. EV (empty vector); mean ± SEM. (B) Correlation between NF-κB induction and cellular expression levels or thermostability by domain using linear regression analysis in GraphPad Prism7. The R234* variant was excluded from correlative analysis. Data provided in Figure 8—source data 1.