Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Homo sapiens) |
TRIM21 | HGNC | HGNC: 11312 | |
| Strain, strain background (Escherichia coli) | C41(DE3) | Sigma-Aldrich | Cat#: CMC0017 | Chemically competent cells |
| Strain, strain background (Human adenovirus type 5) | AdV5-GFP | Viraquest | N/A | |
| Strain, strain background (Human adenovirus type 5/35 chimera) | AdV5/35-GFP | Viraquest | N/A | |
| Cell line (Homo sapiens) |
293T | ATCC | Cat#: CRL-3216; RRID:CVCL_0063 |
|
| Cell line (Homo sapiens) |
TRIM21
Knockout 293T |
Dickson et al., 2018 | DOI: 10.7554/eLife.32660 | CRISPR/Cas9 gene knockout |
| Cell line (Homo sapiens) |
TRIM21
Knockout HeLa |
Bottermann et al., 2019 | DOI: 10.1016/j.chom.2019.02.016 | CRISPR/Cas9 gene knockout |
| Cell line (Homo sapiens) |
Lymphoblastoid cell lines | NHGRI Repository at Coriell Institute for Medical Research | RRID:SCR_004528 | |
| Recombinant DNA reagent | pGL4.32 | Promega | Cat#: E8491 | NF-kB-RE-firefly-luciferase reporter construct (Photinus pyralis) |
| Recombinant DNA reagent | pCR/V1 (HIV-1 Gag-Pol) |
Zennou et al., 2004 | DOI: 10.1128/JVI.78.21.12058–12061.2004 | Lentivirus packaging vector |
| Recombinant DNA reagent | pMD2.G | Addgene | RRID:Addgene_12259 | VSV-G env for making pseudotyped lentiviral vector |
| Recombinant DNA reagent | pHR’ | Demaison et al., 2002 | DOI: 10.1089/10430340252898984 | HIV-1 based lentiviral vector |
| Antibody | Human plasma IgG Fc | Athens Research and Technology | Cat#: 16-16-090707-FC; RRID:AB_575814 |
|
| Antibody | Anti-Human TRIM1 (Mouse monoclonal) | Santa Cruz Biotechnology | Cat#: sc-25351; RRID:AB_628286 |
Immunoblot (1:1000) |
| Antibody | Anti-Human COX IV (Rabbit monoclonal) | LI-COR | Cat#: 926–42214; RRID:AB_2783000 |
Immunoblot (1:5000) |
| Antibody | Anti-Mouse-HRP (Goat polyclonal) | Sigma | Cat#: A0168; RRID:AB_257867 |
Immunoblot (1:5000) |
| Antibody | Anti-Rabbit-HRP (Goat monoclonal) | Sigma | Cat#: A0545; RRID:AB_257896 |
Immunoblot (1:5000) |
| Antibody | Anti-mCherry (Rabbit polyclonal) | Abcam | Cat#: ab167453; RRID:AB_2571870 |
IF (1:500) |
| Antibody | Anti-Human IgG Alexa Fluor 488 (Goat polyclonal) | ThermoFisher | Cat#: A-11013; RRID:AB_2534080 |
IF (1:500) |
| Antibody | Anti-Rabbit IgG Alexa Fluor 568 (Goat polyclonal) | ThermoFisher | Cat#: A-11036; RRID:AB_10563566 |
IF (1:500) |
| Antibody | IRDye 680RD Goat anti-Rabbit IgG | LI-COR | Cat#: 926–68071; RRID:AB_10956166 |
Immunoblot (1:5000) |
| Antibody | IRDye 800CW Goat anti-Mouse IgG | LI-COR | Cat#: 926–32210; RRID:AB_621842 |
Immunoblot (1:5000) |
| Antibody | Humanized anti-AdV5 hexon monoclonal IgG | Foss et al., 2016 | DOI: 10.4049/jimmunol.1502601 | |
| Peptide, recombinant protein | Human TNF-α | PeproTech | Cat#: 300-01A | 10 ng/ml |
| Peptide, recombinant protein | Human IFN-α1 | Sigma | Cat#: SRP4596 | 1000U/ml |
| Sequence-based reagent | Alt-R CRISPR-Cas9 tracrRNA | IDT | Cat#: 1072532 | |
| Sequence-based reagent | Alt-R CRISPR-Cas9 crRNA (TRIM21 KO) |
IDT | N/A | ATGCTCACAGGCTCCACGAA |
| Sequence-based reagent | Alt-R CRISPR-Cas9 crRNA (TRIM21 R118) |
IDT | N/A | GTCACGGTGTTTCCGAGACT |
| Sequence-based reagent | Alt-R CRISPR-Cas9 crRNA (TRIM21 R234) |
IDT | N/A | TCATCTCAGAGCTAGATCGA |
| Sequence-based reagent | ssODN template for HDR (TRIM21 R118Q) | IDT | N/A | CACAGGGGGAACGGTGTGCAGTGCATGGAGAGAGACTTCACCTGTTCTGTGAGAAAGATGGGAAGGCCCTTTGCTGGGTATGTGCTCAGTCTCAGAAACACCGTGACCACGCCATGGTCCCTCTTGA |
| Sequence-based reagent | ssODN template for HDR (TRIM21 R234*) | IDT | N/A | AGCCAGGCCCTACAGGAGCTCATCTCAGAGCTAGATTGAAGATGCCACAGCTCAGCACTGGAACTGCTGCAGGTGAGACAGGGAGGGGTTTCCTTCTACAATTCAGGGAATAACTGAAAAAGACCAG |
| Sequence-based reagent | Forward primer for TRIM21 promoter cloning | Sigma | N/A | ggatcgataagcttgatatcgaattcGCATGTTGTGCACA |
| Sequence-based reagent | Reverse primer for TRIM21 promoter cloning | Sigma | N/A | tgcttaacgcgTGTCAAGTGTGCCGTTAAACAG |
| Commercial assay or kit | Gibson Assembly Master Mix | NEB | Cat#: E2611L | |
| Commercial assay or kit | QIAprep Spin Miniprep Kit | QIAGEN | Cat#: 27106 | |
| Commercial assay or kit | QIAquick PCR Purification Kit | QIAGEN | Cat#: 28104 | |
| Commercial assay or kit | QIAquick Gel Extraction Kit | QIAGEN | Cat#: 28704 | |
| Commercial assay or kit | Neon Transfection System 10 µL Kit | Invitrogen | Cat#: MPK1096 | |
| Software, algorithms | GraphPad Prism 7 | GraphPad | RRID:SCR_002798 | |
| Software, algorithms | R | R Project for Statistical Computing | RRID:SCR_001905 | |
| Other | Hoechst 33342 Solution | Thermo Scientific | Cat. #: 62249 | (1 µg/mL) |
| Other | Steadylite plus Reporter Gene Assay System | PerkinElmer | Cat. #: 6066751 | |
| Other | Fugene 6 Transfection Reagent | Promega | Cat. #: E2691 | |
| Other | Genome Aggregation Database | Broad Institute | RRID:SCR_014964 | |
| Other | 1000 Genomes Project | 1000 Genomes Project | RRID:SCR_006828 |