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. 2019 Oct 15;8:e50069. doi: 10.7554/eLife.50069

Figure 6. Levels of insoluble protein in GRU102.

(A) Silver stain of SDS-Page gel of detergent-insoluble protein, loaded based on five times the volume equivalent of 2 ug soluble protein determined by Bradford protein assay. (B) Silver stain of SDS-Page gel of 0.2 ug detergent-soluble protein determined by Bradford protein assay. (C) Normalized densitometric intensity corrected for differences in protein loading evaluated from soluble protein gel (Densitometric intensity of Gel A/Densitometric intensity of Gel B; Two-way ANOVA Dunnett’s multiple comparisons test, p<0.05, n = 3 repeats for all conditions, each repeat contains approximately 1500 animals collected in independent cohort). Similar result was observed in another independent repeat (Figure 6—figure supplement 1).

Figure 6—source data 1. Protein aggregate levels determined by insoluble gel.
Values were normalized by soluble gel and to Young GRU101.
elife-50069-fig6-data1.xlsx (33.8KB, xlsx)
DOI: 10.7554/eLife.50069.024

Figure 6.

Figure 6—figure supplement 1. Levels of insoluble protein in GRU102 (independent repeat).

Figure 6—figure supplement 1.

(A) Silver stain of SDS-Page gel of detergent-insoluble protein, normalized to total protein in whole-worm lysate. (B, C) Densitometric analysis of the aggregation blot of young and old animals (Two-way ANOVA Dunnett’s multiple comparisons test, p<0.05, *; p<0.005, **; n = 4 repeats for all conditions except for old GRU102 with three repeats, each repeat contains approximately 1500 animals collected in independent cohort).