Author response image 1. Multiple sequence alignment of Mars1 kinase domain (1638-2019 aa) and the five proteins selected by RaptorX as PDB templates to predict Mars1 tertiary structure.

The canonical ATP-binding loop, catalytic triad and EF-helix regions are highlighted by green rectangular frames. The red asterisk indicated the phosphoserine (pS) residue identified in Mars1 activation loop by Mass-Spec analysis. This multiple sequence alignment was generated by ClustalW (Thompson et al., 1994) and its results were visualized using MView (Brown et al., 1998).