Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (C. reinhardtii) | clpP1 | GenBank | L28803.1 | |
| Gene (C. reinhardtii) | VIPP2 | Phytozome | Cre11.g468050 | |
| Gene (C. reinhardtii) | MARS1 | Phytozome | Cre16.g692228 | |
| Recombinant DNA reagent | YFP 3x-FLAG | GenBank | ANF29833.1 | |
| Recombinant DNA reagent | pMJ016c | GenBank | KX077951.1 | |
| Antibody | anti-ClpP1 | gift from Francis-André Wollman and Olivier Vallon |
1:5000 | |
| Antibody | anti-Vipp2 (rabbit, polyclonal) | developed during this study | (1:3000) | |
| Antibody | anti-FLAG (mouse, monoclonal) | Sigma | F1804 | (1:3000 for IB; 1:500 for IF) |
| Antibody | anti-Hsp22E/F (rabbit, polyclonal) | gift from Michael Schroda | (1:10000) | |
| Antibody | anti-DnaK (rabbit, polyclonal) | gift from Jean David Rochaix | (1:10000) | |
| Antibody | anti-α tubulin (rabbit, polyclonal) | Sigma | T5168 | (1:10000) |
| Antibody | anti-Histone H3 (rabbit, polyclonal) | Agrisera | AS10 710 | (1:10000 for IB; 1:500 for IF) |
| Antibody | anti-AtpD (rabbit, polyclonal) | Agrisera | AS10 1590 | (1:500) |
| Antibody | anti-Nab1 (rabbit, polyclonal) | Agrisera | AS08 333 | (1:500) |
| Antibody | anti-AtpD (rabbit, polyclonal) | Agrisera | AS10 1590 | (1:500) |
| Antibody | anti-RpoA (rabbit, polyclonal) | gift from Jean David Rochaix | (1:10000) | |
| Antibody | anti-holo Rubisco (rabbit, polyclonal) | gift from Jean David Rochaix |
(1:10000) | |
| Antibody | anti-Aox1 (rabbit, polyclonal) |
Agrisera | AS06 152 | (1:2000) |
| Antibody | anti-Sultr2 (rabbit, polyclonal) | gift from Arthur Grossman | (1:3000) | |
| Antibody | anti-Hsp90 (rabbit, polyclonal) | Agrisera | AS06 174 | (1:10000) |
| Commercial assay or kit | KOD Hot Start DNA Polymerase | ThermoFisher Scientific | 71086–3 | |
| Commercial assay or kit | Phusion High-Fidelity DNA Polymerase | ThermoFisher Scientific | F530L | |
| Commercial assay or kit | PureYield Plasmid Miniprep System | Promega | A1222 | |
| Commercial assay or kit | NucleoSpin Gel and PCR Clean-up | Clontech | 740609 | |
| Commercial assay or kit | Direct-zol RNA Miniprep Plus | Zymo Research | R2070 | |
| Commercial assay or kit | Alexa Fluor 488 Tyramide SuperBoost Kit, goat anti-mouse IgG | ThermoFisher Scientific | B40941 | |
| Commercial assay or kit | In-Fusion HD cloning plus | Takara | 638910 | |
| Commercial assay or kit | CircLigase II ssDNA | Epicentre | CL9025K | |
| Commercial assay or kit | PrimeScript 1 st strand cDNA Synthesis Kit | Takara | 6110A | |
| Commercial assay or kit | iQ SYBR Green Supermix - Bio-Rad | Bio-Rad | 170–8880 | |
| Commercial assay or kit | Dynabeads kilobase Binder Kit | Invitrogen | 60101 | |
| Commercial assay or kit | Kapa mRNA HyperPrep kit | Roche | KK8540 | |
| Commercial assay or kit | PrepX DNA Library Kit (400075) | Takara | 640101 | |
| Commercial assay or kit | SuperSignal West Femto | ThermoFisher Scientific |
34095 | |
| Commercial assay or kit | SuperSignal West Dura | ThermoFisher Scientific | 34075 | |
| Chemical compound, drug | N6,2′-O-Dibutyryladenosine 3′,5′-cyclic monophosphate sodium salt | Sigma | D0627 | |
| Chemical compound, drug | Thiamine hydrochloride | Sigma | T4625 | |
| Chemical compound, drug | Vitamin B12 | Sigma | V6629 | |
| Chemical compound, drug | digitonin, high purity | Calbiochem | 300410 | |
| Chemical compound, drug | Metronidazole | Sigma | M3761 | |
| Software, algorithm | Sequence Data Analysis | Geneious | ||
| Software, algorithm | Sequence Data Analysis | Snapgene | ||
| Software, algorithm | Microscopy imaging interface | Zeiss | ZEN | |
| Software, algorithm | Image processing | NIH | ImageJ | |
| Other | electroporation system | Biorad | Gene Pulser II | |
| Other | electroporation system | Nepagene | Nepa21 | |
| Other | colony picker | Norgren Systems | CP7200 | |
| Other | colony manipulation robot | Singer | Rotor HAD | |
| Other | Fluorescence scanner | GE Healthcare | Typhoon Trio | |
| Other | Protran NitrocelluloseHybridization Transfer Membrane | Perkin Elmer | NBA083C001EA | |
| Other | Imaging system | LI-COR Biosciences | Odyssey CLx | |
| Other | Plant LED Grow Light | Phlizon 2017 | 2000W | |
| Other | Smart Sensor | Wifi Gateway | G1 | |
| Other | Smart Sensor | Sensor Push | HT1 |