FIG 2.

Gene deletion in SNF-resistant mutants mediated by homologous recombination. (A) Sequence read coverage over a 20-kb region on chromosome 10. Depletion of mapped reads over a region of ∼5 kb was observed in the four mutants compared to the wild-type (WT) L. infantum. A subset of folate-biopterin-transporter genes present on chromosome 10 is shown above the map. Regions of perfect identities between AdoMetT1 (LinJ.10.0370) and LinJ.10.0380 are shown in pale gray. (B) Kinetics of rearrangements at the AdoMetT1 locus with increasing SNF selection (0 μM [lane a], 0.5 μM [lane b], 1.0 μM [lane c], 2.0 μM [lane d], and 50 μM [lane e]). PCR of AdoMetT1 or of the novel junction created by the recombination event (REC) between AdoMetT1 and LinJ.10.0380 were observed using primers shown in Fig. S2C and S2D in the supplemental material. Control PCR was performed with the ptr1 gene.