Figure 4:

FOXP2, NFIA, and NFIB are enriched in the human fetal subplate

(A) Heat maps showing the correlations of module eigengenes (ME) of the NFI factor-containing WGCNA modules built from Brainspan RNA-seq data (Zhu et al., 2018) to each brain region along with significant enrichments of each module with DE genes. For acronym meanings see Table S4B. Cortical regions are written in green. Asterisks represent a correlation with a p-value < 0.01. (B) Heat map showing the mean expression of FOXP2 and NFIA-C and X (Miller et al., 2014) scaled across developmental layer. Marginal zone (MZ), outer and inner cortical plate (CPo, CPi), subplate (SP), intermediate zone (IZ), outer and inner subventricular zone (SZo, SZi), ventricular zone (VZ). (C) T-distributed stochastic neighbor embedding (tSNE) clustering of the single-cell transcriptomes from Nowakowski et al. 2017 (CTX-RG, cortical derived radial glia; MGE-RG/PC, medial ganglionic eminence derived radial glia and progenitor cells; IN, interneuron; IPC/newborn-EN, intermediate progenitor cell/newborn excitatory neuron; ESP, excitatory subplate neuron; EPN excitatory projection neuron; OPC, oligodendrocyte progenitor cell). (D) Expression of FOXP2 and NFIA and NFIB in single-cells. Outlined clusters are significantly enriched for NFIA and/or NFIB expression (Wilcoxon rank sum test with a Bonferroni corrected p-value). (E) Pie charts showing the proportion of all FOXP2-expressing cells (left) or FOXP2-expressing ESP cluster cells (right) that also express NFIA, NFIB, or NFIA and NFIB.