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. Author manuscript; available in PMC: 2019 Oct 15.
Published in final edited form as: Environ Pollut. 2019 Feb 22;248:706–714. doi: 10.1016/j.envpol.2019.02.066

Fig. 2.

Fig. 2

DNA adduct levels measured by 32P-postlabelling in fish gill RTgill-W1 and intestinal RTgutGC cells exposed BaP and 3-NBA for 48 h as assessed. For BaP-derived DNA adducts nuclease P1 digestion, for 3-NBA-derived DNA adducts butanol extraction was used as enrichment procedure. Values represent mean ± SD (n = 4) derived from four independent experiments with cells from different passage numbers. Inserts: Autoradiographic profiles of DNA adducts formed in fish gill RTgill-W1 and intestinal RTgutGC cells after exposure; the origin, at the bottom left-hand corner, was cut off before exposure. B1, 10-(deoxyguanosin-N2-yl)-7,8,9-trihydroxy-7,8,9,10-tetrahydro-BaP (dG-N2-BPDE); N1, 2′(2′-deoxyadenosine-N6-yl)-3-aminobenzanthrone (dA-N6-3-ABA); N2, as-yet unidentified adenine adduct derived from nitroreduction; N3, N-(2′-deoxyguanosine-N2-yl)-3aminobenzanthrone (dG-N2-3-ABA); N4, N-(2′-deoxyguanosin-8-yl)-3-aminobenzanthrone (dG-C8-N-3-ABA).