a Relevant metabolite concentrations for surfactant grown cultures and glucose controls at t0, tm (mid-log), ts (stationary phase), and td (death phase), and statistical significance (p < 0.1) is denoted by (*) next to the measurement. b Proposed surfactant metabolic reconstruction of Halanaerobium congolense WG10 cultured with surfactants compared to glucose controls. Arrow color represents average z-score in proteins from surfactant cultures. Each arrow denotes one biochemical step, whereas each number corresponds to individual proteins. Statistical significance (p < 0.1) for differences in protein relative abundance in surfactant grown cultures are denoted by a black outline around the corresponding arrow. Blacked out arrows indicate the protein was solely found in surfactant grown cultures and not in glucose controls. The protein legend includes the gene number, name, and z-score differences between glucose controls and surfactant grown cultures