Fig. 1.

Major taxa found in the eastern North Pacific drift track. a Taxonomically annotated total community expression over time across size classes. Expression includes only non-organellar ORFs and is normalized by library (time point) within each fraction. Taxa grouping of each ab initio ORF is determined by best LPI hit. Asterisks and tildes denote taxa groups significantly enriched in the large and small size classes, respectively (edgeR, FDR <0.05). b Phylogenetic reconstruction of 16S rRNA gene reference sequences and distributions of active plastids represented in terms of cDNA-based amplicon relative abundances summed across all time points (circles; Supplementary Dataset 5). Circles representing relative amplicon abundance are superimposed over a reference phylogeny which is colored by taxonomy. Proximity of circles to the tips of the branches represents closeness of observed sequences to the references and circle sizes are proportional to normalized read abundance. Note that dinoflagellates known to have tertiary plastids are placed in this method according to the plastid origins (e.g., Gymnodinium myriopyrenoides and several Dinophysis species clading with cryptophytes [93])