Fig. 6.

Elevated aldosterone levels and renin activity in serum of Clcn2^op mice. a–c Serum levels of aldosterone (a), renin activity (b), and corticosterone concentration (c) of Clcn2^+/+ (+/+, circles), Clcn2^+/op (+/op, squares), and Clcn2^op/op mice (op/op, triangles) aged 12 weeks and sub-grouped between males (left) and females (right) (one sample per animal was measured). Aldosterone levels below detection were set at the measureable lower limit threshold value of 70 pM (number of samples below threshold per samples measured: males: Clcn2^+/+ n = 4/7, Clcn2^+/op n = 1/7, Clcn2^op/op n = 0/8; females: Clcn2^+/+ n = 3/7, Clcn2^+/op n = 0/8, Clcn2^op/op n = 0/7). Renin activity was calculated as the sum of angiotensin I (Ang I) and angiotensin II (Ang II) (see Methods). Error bars, mean ± SEM. Statistical significance (Kruskal–Wallis test with Dunn’s multiple comparison test; analyzed separately by sex and compared to Clcn2^+/+) is shown above the groups: **p < 0.01, ***p < 0.001. Upon pooling both males and females in a, statistical analysis (Kruskal–Wallis test with Dunn’s multiple comparison test) of Clcn2^+/+ versus Clcn2^+/op was statistically significant (**) for increased aldosterone