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. 2019 Oct 15;9:14815. doi: 10.1038/s41598-019-51373-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CSF outflow is reduced in glioma-bearing mice. (a) Scheme of experiment timeline. (b) Edema measure (wet/dry ratio) in controls and glioma mice. (c) Saphenous vein signal plot of tracer transport to the systemic blood after cisterna magna infusion of P40D680 (solid line: mean value, dashed line: SD). Control, n = 9; glioma, n = 12. (d) Quantification of transit time to the blood after infusion. Data are the mean± SD. (e) Tracer signal in the blood 60 min after tracer infusion. Data are the mean± SD. (f) Representative images of deep cervical lymph nodes. Scale bar: 1 mm. (g) The mean tracer fluorescent signal of deep cervical lymph nodes 60 min after tracer infusion into lateral ventricle. Data are the mean± SD. (h) Representative images of mandibular lymph nodes. Scale bar: 1 mm. (i) The mean tracer fluorescent signal of mandibular lymph nodes 60 min after tracer infusion into lateral ventricle. Data are the mean± SD.