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. 2019 Jul 4;13(11):2737–2749. doi: 10.1038/s41396-019-0468-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Differences in predicted protein isoelectric points and potassium uptake potential over time. Analysis of the isoelectric points (pI) of proteins encoded in replicates of WMG assemblies from samples harvested at different dates, showing (a) the overall weighted distribution of the protein pIs, and the weighted average pI of proteins encoded in (b) all contigs and (e) only Halobacteria contigs. (d) pI distribution of predicted proteins encoded in Bacteroidetes and Halobacteria contigs. Average potassium uptake potential across time-point samples inferred from trk gene relative abundance and quantified in (c) all contigs and (f) only Halobacteria contigs. Error bars represent standard deviation; significance bars represent group significance based on a two tail t test, and stars denote the p-value thresholds (* = 0.01, ** = 0.001, *** = 0.0001)