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. 2019 Oct 15;8(11):60. doi: 10.1038/s41389-019-0170-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a qPCR analyses of the mRNA expression of HOXA3 following HOXA-AS3 suppression for 48 h. b, c Western blotting detection of the protein levels of HOXA3 following HOXA-AS3 suppression for 48 h. d RNA pull-down assay and western blotting detection of HOXA-AS3 binding to the HOXA3 protein. (L lysate load, FT flow-through; E eluate). e Mass spectrum detection of HOXA-AS3 binding to the HOXA3 protein. f RNA pull-down assay and qPCR detection of HOXA-AS3 binding to the mRNA of HOXA3. (L lysate load, FT flow-through, E eluate). g RNA immunoprecipitation detection of the enrichment of HOXA-AS3 using an anti-HOXA3 antibody. (*p < 0.05, **p < 0.01, ***p < 0.001)