Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Oct 9;10:2313. doi: 10.3389/fmicb.2019.02313

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Xue, Hou, Zhang, Huang, Li, Nan, Mu, Wang, Zhang, Han, Ren, Zhao, Wu, Wang and Zhou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Determination of GP5 domains interact with MYH9 C-terminal region. (A) Predicted topological structure PRRSV SD16 GP5 (top panel) and two ectodomains (Ecto-1 and Ecto-2; bottom panel) and a cytoplasmic domain (Cytodomain) were linked with two GSS linkers and labeled with His-tag to form a truncated GP5 protein (designated GP5Δ-His). (B) The purified GP5Δ-His protein was confirmed with SDS-PAGE and Western blot analysis. (C) Far-Western blot analysis of GP5Δ-PRA interaction. Purified GP5Δ-His (2 μg or 5 μg per lane) and PRA (2 μg, 5 μg, or 10 μg per lane), subjected to SDS-PAGE and transferred to PVDF membrane, were probed with PRA and GP5Δ-His, and then detected with Mab2-5G2 and anti-His mAb, respectively. Recombinant swine hepatitis E virus ORF2 protein (sp239) was used as an irrelevant protein control. (D) Indirect ELISA results of GP5Δ-PRA interaction. Purified GP5Δ-His and PRA protein (200, 400, and 800 ng per well) on the solid-phase of the ELISA plate were incubated with PRA and GP5Δ-His, and following detected with Mab2-5G2 and anti-His mAb, respectively. Data are represented as means ± SD. ^∗∗P < 0.01; ^∗∗∗P < 0.001.