Table 2.
The 10 most abundant, validated human glycosylation sites and their relative abundances in the enriched HeLa cell lysate.
| Glycoprotein | Peptide backbone | Glycosylation site | Relative abundance | High-mannose % | Hybrid % | Complex % | Degree of sialylation % | Degree of fucosylation % |
|---|---|---|---|---|---|---|---|---|
| TFR1_HUMAN | QNNGAFN * ETLFR | 727 | 8.9 | 100.0 | 0.0 | 0.0 | — | — |
| CBPM_HUMAN | NFPDAFEYNN * VSR | 164 | 8.5 | 92.1 | 0.0 | 7.9 | 0.0 | 100.0 |
| LAMP1_HUMAN | GHTLTLN * FTR | 103 | 7.6 | 69.6 | 9.6 | 20.7 | 53.8 | 77.8 |
| FOLR1_HUMAN | NAccSTN * TSQEAHK | 69 | 4.3 | 0.0 | 4.6 | 95.4 | 59.3 | 100.0 |
| DAF_HUMAN | GSQWSDIEEFcN * R | 95 | 4.0 | 0.0 | 0.0 | 100.0 | 91.7 | 8.4 |
| LAMP1_HUMAN | LLNINPN * K | 261 | 4.0 | 23.6 | 35.2 | 41.2 | 3.5 | 84.0 |
| PLOD1_HUMAN | YIHQN * YTK | 538 | 3.9 | 42.6 | 57.4 | 0.0 | 0.0 | 0.0 |
| FOLR1_HUMAN | GWN * WTSGFNK | 161 | 3.7 | 0.0 | 0.0 | 100.0 | 50.0 | 72.8 |
| LAMP2_HUMAN | VQPFN * VTQGK | 356 | 3.2 | 81.0 | 8.6 | 10,4 | 0.0 | 0.0 |
| NICA_HUMAN | IYIPLN * K | 45 | 2.5 | 13.3 | 52.0 | 34.7 | 44.5 | 100.0 |
| Distribution among all validated human glycoforms | 64.9 | 5.1 | 30.0 | 46.8 | 68.7 | |||
Glycosylation site is the position of the glycosylated asparagine in the glycoprotein’s amino acid sequence. Sum of the abundance of all glycoforms corresponding to the given site and the percentage of different N-glycan structure types are also given, together with the degree of sialylation (ratio of sialic acid and galactose residues) and degree of fucosylation (ratio of fucosylated glycoforms compared to the sum of complex and hybrid glycoforms).