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. 2019 Oct 9;10:1248. doi: 10.3389/fpls.2019.01248

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Copyright © 2019 Espley, Leif, Plunkett, McGhie, Henry-Kirk, Hall, Johnston, Punter, Boldingh, Nardozza, Volz, O’Donnell and Allan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) MYB10 activates the promoters of ethylene-associated genes. The promoter sequences from ACS1, ACS3, ACS5, ACO1, and ERF106 were isolated and cloned into the dual luciferase reporter construct and infiltrated into N. benthamiania leaves in combination with MYB10 with and without bHLH3. MYB8 was included as a control. (B) Anthocyanin activating MYBs activate the promoter of ERF106. The promoter sequence from ERF106 was isolated and cloned into the dual luciferase reporter construct and infiltrated into N. benthamiania leaves either on its own or in combination with activating or repressing MYB TFs. Different letters indicate statistical difference with P < 0.05 (7A refers to each promoter independently). Mean of four biological replicates. Error bars show ± SEM.