FIGURE 2.

Both common peroneal and tibial nerve stimulation can induce postsynaptic currents in Shox2 neurons. (A) Cartoon of ventral horn-removed spinal cord preparation with intact sciatic nerve branches for stimulation of common peroneal (CPn) and tibial nerves (Tn). (B) The proportion of excitatory, inhibitory, mixed-E, and mixed-I currents detected in Shox2 neurons following CPn stimulation and following Tn stimulation. (C) Shox2 neurons recorded during stimulation of both CPn and Tn are plotted according to anatomical position and color-coded based on response type. Segment number corresponds to the center point of the dorsal root at the entry zone. Shox2 neurons displaying postsynaptic currents to CPn and Tn stimulation were observed throughout the lumbar cord. (D–F) Latency, duration and amplitude plotted for each response subtype. Mixed-E and mixed-I responses were pooled into a single mixed subtype. Latency and duration did not significantly differ between evoked excitatory, inhibitory or mixed currents, whereas peak amplitude differed significantly between current types (p = 0.027, indicated by star in upper right corner). Post hoc testing did not detect significant pairwise comparisons when corrected for multiple comparisons (see text for details). Amplitude plotted on log scale for clarity.