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. 2019 Oct 9;10:1130. doi: 10.3389/fphar.2019.01130

Figure 3.

Figure 3

SBP extracts potentiate the NGF-induced neurite outgrowth in PC12. (A): Cultured PC12 cells were co-treated with NGF (1.5 ng/ml) together with SBPwater and SBPEtOH, or individual herbal extracts for 48 h. NGF at 50 ng/ml served as a positive control. SBPEtOH, ethanol extracts of various herbal materials and Styrax solution were used at 100 μg/ml; and SBPwater, water extracts of various herbal materials, solutions of Ginseng Radix et Rhizoma extract and Borneolum Syntheticum were used at 500 μg/ml. Scale bar = 100 μm. Representative images were shown. (B): The lengths of neurite were counted as described in Method section. Values were expressed as percentage of cells having different length of neurite in 100 counted cells (total), mean ± SEM, n = 4.