Figure 9.
SBP induces the cAMP-mediated transcriptional activity in PC12 cells. (A): Cultured PC12 cells, transfected with pCRE-Luc, were treated with of forskolin, as indicated for 48 h. The cell lysates were collected to determine the luciferase activity. (B): The pCRE-Luc transfected PC12 cells were treated with SBPwater or SBPEtOH, as indicated for 48 h. (C): The pCRE-Luc transfected PC12 cells were pre-treated with PKA inhibitor, H89 (5 μM), for 3 h, and then treated with or SBPEtOH (100 mg/ml), or SBPwater (500 μg/ml), or forskolin (3 μM) for 48 h. The cell lysates subjected to luciferase assay. Values are expressed as the fold of increase to basal reading, and are in mean ± SEM, n = 4, each with triplicate samples. * p < 0.05; ** p < 0.01; *** p < 0.001.