Table 2.
Virus shedding titers (EID50/ml) in different vaccination regime groups at 3, 5-, and 7-days post challenge using rRT-PCR.
| Vaccination regime (1:14 day old) | Virus shedding titers (EID50/ml)a | ||
|---|---|---|---|
| 3DPC | 5DPC | 7DPC | |
| IB-M41: IB-Var2 | 2.05 ± 0.79*** (5/5)b | 1.61 ± 0.39** (4/5) | 0.81 ± 0.31* (2/5) |
| IB-Var2: IB-Var2 | 1.47 ± 0.20*** (4/5) | 1.19 ± 0.26*** (4/5) | 0.64 ± 0.54* (2/5) |
| IB-Var2: IB-M41 | 1.95 ± 0.66*** (3/5) | 1.60 ± 0.55* (3/5) | 1.16 ± 0.97 (3/5) |
| IB-Ma5: IB-793B | 1.91 ± 0.68*** (5/5) | 1.52 ± 0.67** (5/5) | 1.31 ± 0.64 (4/5) |
| IB-793B: IB-793B | 3.12 ± 0.10** (5/5) | 1.73 ± 0.47** (5/5) | 1.25 ± 0.45 (3/5) |
| IB-793B: IB-Ma5 | 1.67 ± 0.19*** (5/5) | 2.01 ± 0.48* (5/5) | 1.10 ± 0.77 (3/5) |
| Challenge control | 4.07 ± 0.12 (5/5) | 2.93 ± 0.61 (5/5) | 1.90 ± 0.31 (5/5) |
| Negative control | 0 (0/5) | 0 (0/5) | 0 (0/5) |
a
EID50, embryo infective dose fifty; DPC, days post challenge. Asterisks indicate significant differences to the challenge control group (***p ≤ 0.001, **p ≤ 0.01, *p ≤ 0.05).
b
Numbers in parenthesis indicate number of birds tested positive for virus shedding out of tested birds.