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Defibrotide (DF) suppresses endothelial cell (EC) proliferation. An 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay was used to quantify the effect of DF (10, 100, or 500 μg/ml) for (A) 24 h (D + 1), (B) 48 h (D + 2), or (C) 72 h (D + 3) on EC proliferation. Bar diagrams represent the optical density (OD) normalized to control data (%) expressed as the mean ± SEM of three experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
