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. 2019 Oct 9;10:2339. doi: 10.3389/fimmu.2019.02339

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Copyright © 2019 Martinez-Sanchez, Hamelmann, Palomo, Mir, Moreno-Castaño, Torramade, Rovira, Escolar, Cordes, Kalupa, Mertlitz, Riesner, Carreras, Penack and Diaz-Ricart.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Defibrotide (DF) suppresses endothelial cell (EC) tube formation. Representative images of angiogenic activity of ECs by a tube formation assay incubated with DF at different concentrations. Bar diagrams represent different characteristics measured during tube formation: numbers of (A) junctions and (B) master junctions, numbers of (C) segments and (D) master segments, and (E) number, (F) size, and (G) area of meshes, as indicated. Data are expressed as the mean ± SEM of three experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.